Phospholipase C in Living Cells
نویسندگان
چکیده
منابع مشابه
Phospholipase C in Living Cells
We have further tested the hypothesis that receptor-mediated modulation of KCNQ channels involves depletion of phosphatidylinositol 4,5-bisphosphate (PIP2) by phosphoinositide-specific phospholipase C (PLC). We used four parallel assays to characterize the agonist-induced PLC response of cells (tsA or CHO cells) expressing M1 muscarinic receptors: translocation of two fluorescent probes for mem...
متن کاملPhospholipase C in Living Cells: Activation, Inhibition, Ca
We have further tested the hypothesis that receptor-mediated modulation of KCNQ channels involves depletion of phosphatidylinositol 4,5-bisphosphate (PIP 2 ) by phosphoinositide-specific phospholipase C (PLC). We used four parallel assays to characterize the agonist-induced PLC response of cells (tsA or CHO cells) expressing M 1 muscarinic receptors: translocation of two fluorescent probes for ...
متن کاملKinetics of M1 muscarinic receptor and G protein signaling to phospholipase C in living cells
G protein-coupled receptors (GPCRs) mediate responses to external stimuli in various cell types. Early events, such as the binding of ligand and G proteins to the receptor, nucleotide exchange (NX), and GTPase activity at the Galpha subunit, are common for many different GPCRs. For G(q)-coupled M(1) muscarinic (acetylcholine) receptors (M(1)Rs), we recently measured time courses of intermediate...
متن کاملPhospholipase C activation in rat pituitary adenoma (GH) cells.
The presence of the pertussis toxin (PTX) insensitive GTP-binding proteins (C-proteins) G(q) alpha and/or G(11) alpha has been demonstrated in three different prolactin (PRL) and growth hormone (GH) producing pituitary adenoma cell lines. Immunoblocking of their coupling to hormone receptors indicates that G(q) and/or G(11) confer throliberin (TRH) responsive phospholipase C (PL-C) activity in ...
متن کاملTyrosine phosphorylation regulates phospholipase C activation in human T-cells.
Introduction One early transmembrane signal in response to stimulation of the T-cell antigen receptor (CD 3/Ti) is the activation of phosphatidylinositol (Ptd1ns)specific phospholipase C (PLC). The resulting formation of inositol 1,4,5-trisphosphate (Ins( 1,4,5)q?) and diacylglycerol (DAG) causes calcium release from internal stores, calcium influx through calcium channels, and translocation of...
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ژورنال
عنوان ژورنال: Journal of General Physiology
سال: 2005
ISSN: 1540-7748,0022-1295
DOI: 10.1085/jgp.200509309